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Cholesterol CHOD-PAP LS 4×100 ml
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16,203.24 جنيه
Description
FIU_CHOLESTEROL CHOD PAP LS MONO_ENG REV.03-2019By enzymatic hydrolysis, the enzyme Cholesterol Esterase (CHE) frees
Cholesterol from fatty acids. This fraction, together with the free part in the
plasma, is oxidized by enzyme Cholesterol Oxidase (CHO) producing
Hydrogen Peroxide (H2O2). The latter compound, by reaction catalyzed by
the enzyme Peroxidase (POD) reacts with 4-amino-antipyrine to form the
red quinone which can be measured photometrically (Trinder’s reaction).
The measurement of this Absorbance (optical density) is directly
proportional to the cholesterol initially contained in the sample under
examination. The use of a known calibrator makes quantitative analysis
possible.
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