الدهون الثلاثية GPO-PAP LS (4 × 60 مل) 240 ت

ipase (LP) hydrolyzes the Triglycerides to Fatty Acids and Glycerol which, in the presence of Glycerochinase (GK) and ATP, is phosphorylated to Glycerol-3-phosphate and converted by Glycerol-3- phosphate Oxidase (GPO) into Dihydroxyacetonphosphate and H2O2. The formed hydrogen peroxide reacts, in the presence of Peroxidase (POD), with 4-Chlorophenol and 4- Aminoantipyrin, giving a red colored compound whose Absorbance, measured fotometrically at 546 nm, is proportional to the concentration of Triglycerides

Triglycerides      (  LPL)  →  Glycerol + Fatty Acid

Glycerol + ATP       ( GK)→   Glycerol-3-phosphate + ADP

Glycerol-3-phosphate + 02      (GPO) →  Dihydroxyaceton phosphate + H2O2

2 H2O2+Aminoantipyrine+4-Chlorophenol     ( POD)→  Quinone + HCl + 4 H2O

FIU_TRIGLYCERIDES GPO PAP LS MONO_ ENG REV.09-2018