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Optimized UV test according to DGKC (German Society of Clinical Chemistry) and IFCC (International Federation of Clinical Chemistry) for CK with inhibition of CK-M isoenzymes by monoclonal antibodies. CK-MB consists of the subunits CK-M and CK-B. Specific antibodies against CK-M inhibit the complete CK-MM activity (main part of the total CK activity) and the CK-M- subunit of CK-MB. Only CK-B activity is measured, which is half of the CK-MB.
Creatine Phosphate + ADP (CK-B) → Creatine + ATP
ATP + D-Glucose ( HK) → G-6-P + ADP
G-6-P + NADP ( G-6-PDH) → D-Gluconate-6-phosphate + NADPH + H+
Increasing the concentration of NADPH, causes an increase of absorbance of solution, measurable fotometrically at 340 nm, proportionally to activity of enzyme in the sample.1,825.92 جنيه1,825.92 جنيه