Optimized UV-test according to IFCC (International Federation of Clinical Chemistry).

GOT / ASAT catalyzes the transformation of 2-Oxiglutarate and L-Aspartate into L-Glutamate and Oxaloacetate. Subsequently, the Malate- Dehydrogenase (MDH) catalyzes the transformation of the formed Oxalacetate and of the NADH into L-Malate and NAD+ causing a decrease in Absorbance (Abs) of the reaction which is measured at 340 nm. The rate of decrease of the Abs is proportional to the activity of the GOT / ASAT enzyme in the sample.

L-Aspartate + 2-Oxoglutarate    (ASAT (GOT)    →  L-Glutamate + Oxalacetate

Oxalacetate + NADH + H+       ( MDH)    →     L-Malate + NAD+ + H2O

FIU_GOT ASAT IFCC LS 4+1_ ENG REV.03-2019